This previous question was resolved after re-reading my FAQ:
http://www.experts-exchange.com/Apple/Hardware/iPhone/Q_25018359.html
Closing comment was:
Author Comments:This ended up being the issue. I was basically being an idiot and not following Alan's instructions. He helped me out and fixed it..thanks to Alanhardisty for all the help.
Can you please re-read my article, check the IIS settings very carefully, then if you make any changes, run iisreset and then re-test.
Is your server running Small Business Server?