Try adding whereclauses as below into the queries
INSERT INTO tblDept ( DeptID, Department )
SELECT tblDept2.DeptID, tblDept2.Department
FROM tblDept2
WHERE tblDept2.DeptID NOT IN (SELECT tblDept.DeptID FROM tblDept)
INSERT INTO tblJobs ( JobID, Title )
SELECT tblJobs2.JobID, tblJobs2.Title
FROM tblJobs2
WHERE tblJobs2.JobID NOT IN (SELECT tblJobs.JobID FROM tblJobs)
OR run DELETE statements prior to running the inserts
DELETE * FROM tblDept;
DELETE * FROM tblJobs;